The site on a new location! Should you have made a bookmark in
the past (earlier than March 1st 2005) please delete this one and make a new
one. With this new location we will be able to update the site again and add the
new pages as we promised already for a long time.
Hopefully the subjects like 'staining of nuclear
factors' and 'the tracking of two dividing populations of living cells' simultaneously
will be in the site very soon now.We will try to keep the site
up to date. All your suggestions
and papers to revere to are welcome.
I would like to thank everyone that
contributed in the last years. Hopefully we can help more and more people in the
On the site we
give some examples, protocols and hints for the detection of
intracellular antigens at the single cell level using flow cytometry or fluorescence microscopy.
Our special interest is the detection of cytokines at the single cell
this topic will be dealt with extensively.
You will also find some related
techniques and alternatives. Like the isolation of living specific cytokine producing cells by means of
the cytokine secretion assay and of the tracking
of cell division by using CFDAse.
An other community you can also put your questions concerning flow cytometry to is the Purdue Cytometry
List. A lot of
experts is subscribed to this list and they try to help each other when problems
Enter this site and please don't hesitate to ask us questions, give us some
advise or protocols to add or point out errors. We hope to be of some help,