Welcome Proliferation Cytokines IC Enzymes IC Signal transduction Symposia



The site on a new location! Should you have made a bookmark in the past (earlier than March 1st 2005) please delete this one and make a new one. With this new location we will be able to update the site again and add the new pages as we promised already for a long time.
Hopefully the subjects like 'staining of nuclear factors' and 'the tracking of two dividing populations of living cells' simultaneously will be in the site very soon now.We will try to keep the site up to date. All your suggestions and papers to revere to are welcome.

I would like to thank everyone that contributed in the last years. Hopefully we can help more and more people in the future.

On the site we give some examples, protocols and hints for the detection of intracellular antigens at the single cell level using flow cytometry or fluorescence microscopy. 
Our special interest is the detection of cytokines at the single cell level. And this topic will be dealt with extensively.
ou will also find some related techniques and alternatives. Like the isolation of living specific cytokine producing cells by means of the cytokine secretion assay and of the tracking of cell division by using CFDAse.

An other community you can also put your questions concerning flow cytometry to is the Purdue Cytometry List. A lot of experts is subscribed to this list and they try to help each other when problems rise.

Enter this site and please don't hesitate to ask us questions, give us some advise or protocols to add or point out errors. We hope to be of some help,

Joost Schuitemaker

Although we did our best we can not be held responsible for any mistakes or typing errors.
 Suggestions, literature and protocols are welcome at icstaining@schuitemaker.info. Copyright 2000 - 2005.
Page was last updated: 05-03-2005