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Nuclear factors and flow cytometry are not the best combination. Often the
hypothesis asks for localization of these factors and than the only protocol
that can answer the question is that where a laser confocal microscope is used.
In some situations though it is enough to evaluate the phosphorylation levels of
certain factors. Than a (phospho) specific antibody might answer the question in
combination with surface markers. For instance when memory and naive T cells
subsets are evaluated for the expression of phosphorylated STAT factors. Papers
are published in the last few years showing specific staining with the use of
flow cytometry.
Translocation of factors, like NFkB subunits, (confocal)
microscopy is necessary. In the coming months we will add a new part concerning
this technique. Does it take to long for you..... please contact us.
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Signal transduction
