Signal transduction

Welcome Proliferation Cytokines IC Enzymes IC Signal transduction Symposia







Nuclear factors and flow cytometry are not the best combination. Often the hypothesis asks for localization of these factors and than the only protocol that can answer the question is that where a laser confocal microscope is used.

In some situations though it is enough to evaluate the phosphorylation levels of certain factors. Than a (phospho) specific antibody might answer the question in combination with surface markers. For instance when memory and naive T cells subsets are evaluated for the expression of phosphorylated STAT factors. Papers are published in the last few years showing specific staining with the use of flow cytometry.

Translocation of factors, like NFkB subunits, (confocal) microscopy is necessary. In the coming months we will add a new part concerning this technique. Does it take to long for you..... please contact us.


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Page was last updated: 05-03-2005