IL-10 One of the cytokines that is looked for in T lymphocyte immunology at the moment is IL-10. This cytokine is playing an important role in the regulation of the immune response. And it is very difficult to detect it intracellularly. The reports about this difficulty in detection are very heterogeneous. Some state it is not that difficult, some state it is impossible. In our experience it seem to be the (T) cells that are the most important factor in being able to detect IL-10.
IL-12 Also the detection of IL-12 is a special point of attention. We go into this under dendritic cells.
IL-18 The same results can be obtained by the search for the cytokine IL-18. We regret the fact that there is only one company in the world that has the right to sell anti-human IL-18. And they do it at a price that is unrealistic. Many of us make the choice not to look into this cytokine.
TGFß for instance is a molecule that is still difficult to detect. It is said (and published by M.L. Garba and J.A. Frelinger of UNC Chapel Hill, USA) that the clone TB21 is able to detect TGFß. We did test this antibody and had no luck in detecting TGFß in PBMC. We have found no other references than Garba et al. or other antibodies that show intracellular staining for TGFß.
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