Cell source

Cell source Stimulation Secretion stop Fixation Permeabilisation Staining Protocol




In general every cell type can be used in the saponin protocol. The only condition to look at the cells in a flow cytometer is a single cell suspension. But cells like erythrocytes will not hold during the procedure. In the saponin step these cells will lyse. Single cell suspensions are a pre but the protocol will also work on slides with sheets of cells, like epidermis. These will be evaluated with use of a microscope instead of the flow cytometer.
Since the start of this site the protocol has been used for almost all types of cells. At the literature page you will a selection of articles.

Although we did our best we can not be held responsible for any mistakes or typing errors.
 Suggestions, literature and protocols are welcome at icstaining@schuitemaker.info. Copyright 2000 - 2005.
Page was last updated: 05-03-2005