| Wednesday, May
29th:
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| 09.00-17.00 |
Symposium ‘Faces
and phases of dendritic cells’
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| Thursday, May 30th
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|
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| 08.30-10.00 |
Introduction |
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| 10.00-18.00 |
1. Isolation of blood dendritic
cells by MACS technique. |
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2. Phenotyping of blood dendritic cells by FACS. |
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3. Stimulation of (dendritic) cells for intracellular staining of
cytokines.
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4. CFSE staining of cells. |
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|
| 20.00 |
Workshop diner |
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Friday, May 31th
|
|
|
| 08:30-10:00 |
Introduction |
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|
| 10.00-18:00 |
1. Intracellular staining and
evaluation of cytokines (dendritic cells). |
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2. Evaluation of CFSE in combination with intracellular cytokine staining (T
cells). |
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3. Cytokine Secretion Assay; the isolation of living
cytokine-secreting T cells. |
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|
| 18:00-19:30 |
Evaluation workshop/snack |
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Program
