It is very difficult to give combinations of fluorochromes that will work on a
specific cytometer (or microscope). This is all depending on the set of filters and the laser in
the machine. The manufacturer always gives a suggestion with the machine. Try
to get the protocol up and running and when preferred look for alternatives than. Most of the
time your representative will help you with suggestions.
In the excitation/emission table you may find a suitable
alternative. Most important is the wavelength of your laser and the wavelength
cut off of the mirrors in the machine. It is possible to get this
information from the manufacturer.
Although we did our best we can
not be held responsible for any mistakes or typing errors.
Suggestions, literature and protocols are welcome at
icstaining@schuitemaker.info.
Copyright 2000 - 2005.
Page was last updated:
05-03-2005
Combinations
